Abstract: In order to establish the quantitative detection method of E.coli O157:H7 in foods by using double antibody sandwich ELISA,the IgY produce by chicken against E.coli O157:H7 specific lipopolysaccharide was used as capture antibody,and the HRP-IgY was used as detection antibody. In the conclusion,when the quantity of E.coli O157:H7 in foods is more than 104 CFU/ ml,it can be dectect directly by double antibody sandwich ELISA,but when the quantity of E.coli O157:H7 in foods is less than 104 CFU/ml,the food must enrich the bacterium for 14 h,and then be detected by double antibody sandwich ELISA.

Key words: E.coli O157:H7, foodborne pathogenic microorganism quantitative detection, double antibody sandwich ELISA