Abstract: A simple and sensitive method was established for the separation and detection of 6 organic acids (oxalic acids,tartaric acid,malic acid,formic acid,succinic acid and acetic acid) by nonsuppressed ion exclusion chromatography with conductometric detection. The effect of benzoic acid concentration,flow rate and column temperature on the separation of organic acids was investigated by using Ion Pac ICE-AS6 as analytical column and diluted benzoic acid of lower background as eluent. Through the test,the optimal separation of six organic acids was achieved with 1.0mmol/L benzoic acid solution as the eluent at a flow rate of 0.45 ml/min,and column temperature at 35℃. Detection limits for the organic acids ranged from 0.0341 to 0.9045 mg/L,the relative standard deviations were between 0.13% and 5.12%,and the recovery rates were between 90.75% and 108.5%,the measuring method was provided with better accuracy and precision. The organic acids in 23 Jiangxi wine samples from the five different manufacturers were analyzed with satisfactory results.

Key words: Ion-exclusion chromatography, organic acids, wines