Abstract: In this study,the optimal ultra-sonic extraction conditions of tea saponin(TS) are determined as below:ethanol concentration 80%,ratio of material-liquid 1:4,extraction temperature 40 ℃,ultra-sonic powder 320 W,and extraction time 60 min.Under these conditions,the yield of crude TS is 18.54%,and the content of TS is 60.3%.Then the crude TS was further purificated and separated by silica gel column.The results showed that silica gel chromatograph can efficiently separated TS from impurities,which is suitable to purificate TS.

Key words: tea saponin, extraction, ultra-sonic, silica gel chromatograph, purification