FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (11): 300-304.

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Effects of Different Organic Solvents Combined with Enzymatic Treatments on Permeability of Aperture of Rape Seed Pollen

 DONG  Jie, ZHANG  Hong-Cheng, LI  Chun-Yang, LI  Hui-Yan   

  1. 1.Institute of Apicultural Research,Chinese Academy of Agricultural Science,Beijing 100093,China;2.National Research Center of Bee Product Processing,Ministry of Agriculture,Beijing 100093,China;3.Institute of Agro-food Science and Technology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China
  • Online:2008-11-15 Published:2011-12-08

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Abstract: Rape seed pollen was firstly pretreated with different organic solvents and then treated with different enzymes to improve the permeability of its aperture.The experimental results indicated that after the treatment with medium temperature α -amylase(BAN,Bacterial Amylase Novo) and cellulase(Celluclast,Novozyme),the soluble sugar content in the supernatant of 1 g rape seed pollen treated with petroleum ether is 0.354±0.054 g,increasing by 40% compared with the control sample.After the treatment with protease(Protamex) and cellulase(Celluclast),the protein solution index of rape seed pollen treated with petroleum ether is up to 0.76±0.04,and it was observed that the aperture of the rape seed pollen opens more greatly under microscope.As a conclusion,the aperture of the rape seed pollen is improved obviously.

Key words: rape seed pollen, organic solvents, enzymatic treatment, aperture