FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (11): 87-89.

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Study on Interaction of Thiamine with Tartary Buckwheat Storage Protein

 ZHANG  Xin, CUI  Xiao-Dong, WANG  Zhuan-Hua   

  1. Key Laboratory of Chemical Biology and Molecular Engineering,Ministry of Education,Institute of Biotechnology,Shanxi University,Taiyuan 030006,China
  • Online:2008-11-15 Published:2011-12-08

Abstract: A storage protein from tartary buckwheat seeds was purified by salting out,anion exchange chromatography and size exclusion chromatography.The interaction of thiamine with the target protein was studied by fluorescence spectroscopy.The quenching mechanism is suggested as static quenching caused by formation of new compound.The equilibrium constant is 3.9×104 L/mol.The number of binding site is 1.

Key words: tartary buckwheat, thiamine-binding protein, fluorescence spectroscopy