Abstract: An analytical method of gas chromatography with flame ionization detection after precipitation with Carrez reagent was developed and validated for determination of sodium cyclamate in moon cake matrix. The effects of Carrez reagent amount on determination of sodium cyclamate and the extraction efficiencies of homogenization,ultrasonic and grinding methods were extensively investigated. After homogenization,samples were cleaned-up with Carrez reagent and derived with nitrite sodium and sulphuric acid,and the cyclohexyl nitrite was analyzed by using gas chromatography with flame ionizator detector. At 0.25,0.5 and 1.0 mg/g fortification levels,the average recoveries range from 85.12% to 99.38% with the relative standard deviations from 6.44% to 8.31%. The method shows good linearity over the range assayed 0.125 to 1.0 g/L and the detection limit is 2 mg/kg. It is suitable for determination of sodium cyclamate in complex matrix food with rapidity,sensitivity and accuracy.

Key words: gas chromatography, complex matrix, moon cake, sodium cyclamate, sample preparation