Designing and Application of Test Strip for Fast Detection of Nitrate in Food Based on Enzymatic Method

Abstract

Abstract: Objective:A test strip for fast detection nitrate in food was designed based on enzymatic method and applied in several vegetables. Methods:With 2.0% N-1-naphthylethylenediamin dihydrochloride,3.0% sulfanilic acid and 6.0% tartaric acid mixed by the volume 1:1:1 as the color-developing reagent,qualitative filter paper used for the test strip carrier and nitrate reductase used for the reductant,the test strip was made successfully and used to detect nitrate levels in several vegetables compared with national standard method of China. Results:the color-developing reagent and nitrate reductase could react for fast detection of nitrate. Conclusion:The designed test strip can be used to detect nitrate in food,with higher precision and sensitivity,smaller volume and lower cost,therefore it would have huge potential for development.

Key words: nitrate, test strip, nitrate reductase, fast detection

SUN Jing-Xin, LI Feng-Mei. Designing and Application of Test Strip for Fast Detection of Nitrate in Food Based on Enzymatic Method[J]. FOOD SCIENCE, 2008, 29(12): 575-578.

[1]	LI Cuihong, WEI Lijuan, MU Yuwen, FENG Yuqin. Effect of Combined Treatment with Ice Water and Lysozyme on Spinach Quality and Nitrate Content [J]. FOOD SCIENCE, 2020, 41(7): 203-209.
[2]	PENG Yong, WANG Junxiao, MA Yurong, LIU Pei, WANG Qingguo. Inhibitory Effect of Prohexadione-Calcium Combined with Calcium Nitrate on Bitter Pit of Fuji Apple [J]. FOOD SCIENCE, 2020, 41(3): 205-211.
[3]	ZHANG Wei, ZHANG Wenzhong, GUO Ping, HU Zhongyi, Lü Xiaoli. Evaluation of Commercial Rapid Test Strips for Detection of Aflatoxin B1 in Edible Oil [J]. FOOD SCIENCE, 2020, 41(12): 326-331.
[4]	CHEN Chunyang, ZHANG Chenning, SHI Aiying, DU Xinjun, WANG Shuo. Rapid Detection of Cronobacter sakazakii Using Recombinase Polymerase Amplification Combined with Lateral Flow Strip [J]. FOOD SCIENCE, 2019, 40(24): 306-312.
[5]	LI Yanhua, YANG Yun, XU Weihong, ZHOU Xinbin, WANG Weizhong, CHI Sunlin, LI Tao, ZHANG Chunlai, ZHAO Wanyi, QIN Yuli, WANG Zhengyin, XIE Deti. Nitrate Content, Activities of Key Enzymes for Nitrogen Metabolism, and Expression and Subcellular Localization of NRT1 in Different Nitrate-Enriched Varieties of Pakchoi (Brassica chinensis L.) [J]. FOOD SCIENCE, 2018, 39(7): 78-84.
[6]	CHANG Chao, WANG Ling, WU Jin’e,. Development of a Bioluminescence Method Combined with ATP Amplification for Detection of Bacteria in Dairy Products [J]. FOOD SCIENCE, 2018, 39(4): 320-324.
[7]	LI Xiuming, MA Lizhen. Simultaneous Determination of Nitrate and Nitrite in Vegetables and Meat Products by High Performance Capillary Electrophoresis [J]. FOOD SCIENCE, 2018, 39(12): 301-307.
[8]	LIU Nian, CHEN Pei, GAO Bing, WANG Chao, XU Ning, HU Yong, SHI Wenbo, LI Dongsheng, ZHOU Mengzhou. Progress in Understanding Degradation of Nitrite by Lactic Acid Bacteria [J]. FOOD SCIENCE, 2017, 38(7): 290-295.
[9]	ZHOU Binbin, ZHANG Jihong, WANG Fangbin, WANG Xiali, DAI Xuan, YANG Tao, HAO Yuanqiang. Design, Synthesis and Application of Colorimetric Probe for Cyanide Detection in Food [J]. FOOD SCIENCE, 2017, 38(12): 304-309.
[10]	MA Liang, ZHANG Yuhao, JIANG Tao, SU Min, TU Chunrong. Rapid Detection of Aflatoxin M1 by Ternary Supramolecular System-Based Fluorescence Enhancement [J]. FOOD SCIENCE, 2016, 37(24): 197-202.
[11]	ZHENG Xiaofeng, HAN Yong, LIU Yingyu, ZHANG Juntao, ZHANG Qi, ZHANG Xiaohong, YAO Gang. Preparation and Applicability of a Simple and Fast Test Strip to Detect Listeria monocytogenes [J]. FOOD SCIENCE, 2016, 37(20): 191-197.
[12]	SUN Zhonglei, XU Yi, LI Yu, ZHAO Liyu. Rapid Determination of Nitrate Content in Pickled Mustard Tuber by Ion-Selective Electrode Method [J]. FOOD SCIENCE, 2016, 37(14): 184-188.
[13]	CHEN Yongqin, FENG Bo, XU Weihong, YANG Yun, CHI Sunlin, CHEN Xugen, WANG Zhengyin, XIE Deti. Relationship between Nitrate Contents of Chinese Cabbage (Brassica chinensis L.) and Light Intensity or Key Enzymes of Nitrogen Metabolism [J]. FOOD SCIENCE, 2016, 37(13): 183-188.
[14]	CHI Sunlin, YANG Yun, XU Weihong, CHEN Xugen, CHEN Yongqin, XIE Wenwen, XIONG Shijuan, WANG Zhengyin, XIE Deti. Relationships between Nitrate Contents and NO3-/NH4+ Ratio or Key Enzymes of Nitrogen Metabolism in Bok Choy (Brassica rapa var. chinensis) [J]. FOOD SCIENCE, 2015, 36(23): 70-77.
[15]	ZHANG Jinyan, XIONG Yan, LIAO Qiegen, LI Weihong, WANG Donggen, ZHANG Li, LUO Linguang. Development of CdTe Quantum Dots-Based Lateral-Flow Immunoassay for Rapid Detection of Clenbuterol [J]. FOOD SCIENCE, 2015, 36(22): 161-164.

Designing and Application of Test Strip for Fast Detection of Nitrate in Food Based on Enzymatic Method

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments