Analysis of Phosphatidylcholine Content in Soy Lecithins with Thin-layer Chromatographic Scanner

Abstract

Abstract: The content of phosphotidylcholine in soy lecithins was determined by using dual wavelength thin-layer chromatographic scanner. The determination was conducted under the conditions of chloroform-ethanol-acetic acid-water(40:9:3:1.5,V/V) as developing solvents on a TLC silica palata,scanning wavelength 380 nm,referance wavelength 354 nnm,and dual wavelength reflection zigzay sanning the peak area of phosphatidylcholine. The results showed that the relative standard deviation(RSD) of stability is 3.20%,and the RSD of recovery rate is 3.26%. The method is simple and economical with good repetition and stability. So it could be applied in for the determination lecithin compounds.

Key words: lecithin, phosphotidylcholine, thin -layer chromatography, dual wavelength

SHAO Rong, WU Li-Qin, YUN Zhi. Analysis of Phosphatidylcholine Content in Soy Lecithins with Thin-layer Chromatographic Scanner[J]. FOOD SCIENCE, 2008, 29(12): 618-621.

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Analysis of Phosphatidylcholine Content in Soy Lecithins with Thin-layer Chromatographic Scanner

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