Determination of Cholesterol Content in Egg Powder with High-performance Liquid Chromatography-Mass Spectrometry

Abstract

Abstract: An isotope dilution mass spectrometry(IDMS) method was developed for determination of cholesterol in egg powder,using high-performance liquid chromatography with atmospheric pressure chemical ionization coupled to MS(HPLC-APCI-MS). The cholesterol and its isotope were detected in selected ion monitoring(SIM) model,and the traceability chain was established by the [3,4-13C] cholesterol. The method has high accuracy and precision,can may be used as a candidate reference method.

Key words: isotope dilution mass spectrometry(IDMS) method, cholesterol, egg powder, traceability

SHENG Ling-Hui, WANG Jing, WU Li-Qing, GAO Yun-Hua, FU Bo-Qiang, ZHAO Zheng-Yi. Determination of Cholesterol Content in Egg Powder with High-performance Liquid Chromatography-Mass Spectrometry[J]. FOOD SCIENCE, 2008, 29(12): 632-634.

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Determination of Cholesterol Content in Egg Powder with High-performance Liquid Chromatography-Mass Spectrometry

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