Abstract: Perilla frutescens(linn) Britt seed oil(PO) was extracted with supercritical-CO2 method. This study aimed to evaluate the effects of PO on nonalcoholic fatty liver. The results showed that the PO significantly lowers the activities of aminotransferase(AST),and alanise aminonsferase(ALT),and reduces the contents of malondialdeh1yde(MDA),total Cholesterol(TC),triacylglycerol(TG),low density lipoprotein cholesterol(LDL-c),the peroxidation product,malondialdehyde(MDA) and liver indexes and elevates the activity of superoxide dismutase(SOD) and high density lipoprotein cholesterol(HDL-c) in serum and liver of male Wistar rats. Histological examination is consistent with physiological indicators. In conclusion the PO can be proposed to protect the liver against fatty liver in rats,and the protective effect on liver may be correlated with its antioxidant and free radical scavenger effects.

Key words: supercritical-CO2 extraction, GC-MS, Perilla frutescens(linn) Britt seed oil, nonalcoholic fatty liver