FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (12): 99-101.

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Study on Activities in vitro of Apiin

 JIA  Jia, XIE  Jing-Li, XU  Xue-Shu   

  1. 1.State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China;2.Shandong Silk Textile Vocational College,Zibo 255300,China
  • Online:2008-12-15 Published:2011-12-08

Abstract: Leaf of Apium graveolens L. var. dulce is rich in flavonoids. The refined extract was obtained by means of successive extraction,silica gel column chromatography and recrystallization. According to identification results with HPLC-ESI-MS,the refined extract mainly consists of apiin with 93 % of purity. The total antioxidant activity of the refined extract is 1.1 U/μg,SC25(the quarter-maximal scavenging concentration) for scavenging nitrite is 16.53μg/ml and SC50(the half-maximal scavenging concentration) for scavenging ferrous is 8.53 μg/ml. Moreover,it can enhance the stability of lipids and inhibit lipid free radicals,and the protection factors(PFs) to bean oil and lard are 2.13 and 2.14,separately.

Key words: leaf of Apium graveolens L. var. dulce, apiin, nitrite, ferrous, lipid free radicals