FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (3): 207-210.

Previous Articles     Next Articles

Study on Membrane Separation Technology of Ovotransferrin from Egg White

 ZHANG  Tie-Hua, YIN  Yong-Guang, ZHANG  Ting, LIU  Jing-Bo   

  1. 1.College of Biology and Agriculture Engineering, Jilin University, Changchun 130001, China; 2.College of Quartermaster Technology, Jilin University, Changchun 130062, China
  • Online:2008-03-15 Published:2011-08-24

Abstract: By microfiltratio,ultrafiltration and nanofiltration technology, and combining freeze-drying technology ovotransferrin(OVT) was extracted from egg white. Results showed that there is suitable liquid viscosity of egg white by 5 times normal sodium deliquation, 20 ℃ and 40 MPa homogen handling. After microfiltratio removing impurity, the big member proteins are removed through 10 kD ultrafiltration membrane, and then continuously through 5kD ultrafiltration membrane. Freeze-drying thick liquid obtained is OVT after nanofiltration desalination concentrate.The relative member quality of OVT is 80 kDa by SDS-PAGE, and OVT purity is 28.46%.

Key words: egg white, membrane separation technology, ovotransferrin(OVT), sparation, etraction