Abstract: The β-glucan was extractted from naked oat bran, the effect of different pretreatment method, enzymatic removal of starch, isoelectric precipitation of protein residue, and precipitation of water-soluble polysaccharides with ethanol solution on the yield and purity of β-glucan were systematic studied. From the single factor and orthogonal test, the optimum extracting conditions were fixed.The preparation produced 90% β-glucan.

Key words: naked oat bran； &beta, -glucan； extraction； optimization；