Abstract: Objective: To develop a high performance medium for isolating Klebsiella. Method: Based on the character of Klebsiella that ferments inositol, and adonitol and is tolerant of carbenicillin, developing Maconkey-Inositol-Adonitol-Carbenicillin (MIAC) medium, observing the colonies of 33 strains of reference on different separating media, and assessing the proliferating effect and the practice appliance. Results: Klebsiella forms characteristic red colonies (diameter of 2～4 mm) on MIAC plate. The proliferating assessment indicated that MIAC had no significant difference with nutrient agar(result of rank sum test, p>0.05). Practice appliance indicated that MIAC had a high specificity, and simplified the lab work. Conclusion: MIAC can be used to isolate Klebsiella.

Key words: Klebsiella, isolating medium, MIAC