Abstract: Determination of flavonoids in Qianshan Acanthopanax senticosus leaves and fruits by three-wavelength spectrophotometry can eliminate the absorbance error of interfering components, turbid solution and the scattering effect. Back-ground changed with concentration changed and asymmetric absorption peak problems can be solved also. The regression equation of concentration ΔA was obtained: ΔA =88.795C-0.2060 with a relation coefficient), r=0.9994. The experimental results demostmte the total flavonoids concentrations in 0～40.6 6μg/ml withΔA obeying linear relation. When the absorbanee was measured at wavelength λl=463 nm, λ2=417 nm andλ3=382 nm. The recovery is 96.86%～102.46% and the coeficient of variation is 0.136%. The method is more advantageous than tranditional spectrophotometry method.

Key words: flavonoids, Qianshan Acanthopanax senticosus, leaves, fruits, three-wavelength spectrophotometry