Abstract: Fresh carrot, tomato and chili were used as test samples, absolute alcohol were used in process of triturating and dehydrating, acetone were used for extraction, all of the alcohol solution and acetone solution were commixed, and the content of β-carotene were directly determined by HPLC. The results indicated that: The content of β-carotene in fresh carrot, tomato and chili were 0.841, 0.067, 0.260 mg/100 g, respectively;The recovery test ofβ-carotene were 95.45%(RSD=1.41%, n=5), 97.17%(RSD=0.94%, n=5), 97.54%(RSD=0.86%, n=5), respectively;This method is accurate, convenient and reproducible, and can be used for determination of β-carotene in other fresh fruits or vegetables.

Key words: fresh fruits and vegetables； carrot； tomato； chili； &beta, -carotene； rapid determination；