Study on Separation,Purification and Immune Activity of Peptide from Bee Pollen

FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (3): 450-452.

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Study on Separation,Purification and Immune Activity of Peptide from Bee Pollen

SUN Pei-Long, ZHANG Yi-Min, HE Rong-Jun, YANG Kai

College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China

Online:2008-03-15 Published:2011-08-24

Abstract

Abstract: There are many kinds of proteins and peptides in the bee pollen. Many of them can significantly enhance immunity. A peptide was isolated and purified from the crude bee pollen proteins which was salted out by 60%～80% ammonium sulfate. The molecular weight estimated from SDS-PAGE was 36.3 kD. The purification procedures included Superose-12 chromatography and DEAE Sepharose F.F. ion-exchange chromatography. And the peptide could significantly enhance immunity through the mice test.

Key words: bee pollen, purification, peptide

SUN Pei-Long, ZHANG Yi-Min, HE Rong-Jun, YANG Kai. Study on Separation,Purification and Immune Activity of Peptide from Bee Pollen[J]. FOOD SCIENCE, 2008, 29(3): 450-452.

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Study on Separation,Purification and Immune Activity of Peptide from Bee Pollen

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