Abstract: The experiment is designed to study the extraction technology of isoflavones from soybean residue. According to the solo factors and the orthogonal test. It showed that the optimum technological conditions was the best temperature 80℃, the solid-liquid ratio 1:20, the total time 2 h, the best proportion of ethanol 60%. The extraction yield could be up to 0.59%. Besides, according to the results of the static adsorption and desorption of macroporous resin, results showed that the best macroporous resin is D4006 or H103. After purified by D4006, the purification of isoflavones could be up to 30.58%.

Key words: isoflavones, extraction, purification, macroporous resin