Comparison of Determination of Theanine in Different Tea Species

Abstract

Abstract: High performance liquid chromatography (HPLC) was used for the determination of theanine in different tea species. Theanine can be separated on a Eclipse XDB-C18 column with trifluoroacetic acid (0.05%) as solution and methanol as mobile phase for gradient elution. The UV detection wavelength is set at 208 nm, the flow rate 0.8 ml/min. and the temperature of column 30 ℃. Under the conditions mentioned above, the relative peak area(Y) is linearly related with the concentration(X) of theanine and its linear regression equation is Y=4299.3 66X+14.828, R2=0.9997. The average recovery is 101.06%.The RSD is <0.5%. The method is simple and accurate, and can be used in determination of the black tea, green tea, sweet tea and most of the tea species.

Key words: theanine, tea, high performance liquid chromatography

ZHANG Jian, RONG Shao-Feng, GONG Gang-Ming, QIU Zi-Chun. Comparison of Determination of Theanine in Different Tea Species[J]. FOOD SCIENCE, 2008, 29(4): 335-337.

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Comparison of Determination of Theanine in Different Tea Species

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