FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (4): 344-346.

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Study on LC-ESI-TRAP-MS Determination of Chloramphenicol, Thiamphenicol and Florfenicol in Milk Products

 LIU  Yan-Qin, WANG  Hao, YIN  Xiao-Yan, YANG  Hong-Mei, YAN  Long-Bao   

  1. Haidian District Institute of Products Quality Supervision and Inspection
  • Online:2008-04-15 Published:2011-08-24

Abstract: This study developed a LC-ESI-TRAP-MS method for eht respective determination of chloramphenicol, thiamphenicol and florfenicol in milk products.The mass-spectrometer was operated in the negative ion mode with multiple reaction monitoring. chloramphenicol, thiamphenicol and florfenicol could be qualitatively and quantitatively determined respectively with this method at the same time. The limit of detection is 0.3μg/L and 1.0μg/L, and the limit of quantitation is respectively 0.3~ 1.1 μg/L in milk and 3.0~11.0μg/kg in powdered milk. The linear plots were obtained between 1.0μg/L and 30.0 μg/ L.Overall recoveries were between 77.9 % and 96.6 %, and RSD value were 7.7 % and 14.0 %. The advantages of this method are as follows: simplified operation, shorter operation time, and higher sensitive.

Key words: LC-MS, chloramphenicol, thiamphenicol, florfenicol, residue