Study on Extraction and Determination of γ-Aminobutyric Acid in Tea

Abstract

Abstract: The method has been studied to extractisolate and determine the γ-aminobutyric acid in tea. Taking water or alcohol as solvent, it was discovered that water can achieve a better effect with, γ-aminobutyric acid content to be enhanced 20 %. With the ion exchange resin for isolation and 732 positive ions resin columns (2.5×50 cm) for adsorption, and citric acid and ammonia water as buffer liquids, so as to carry on pH linear gradient elution the results indicated that the γ-aminobutyric acid concentration can reach roughly 39 %. By thin layer scanner to continue the mensuration, the assaying conditions and parameters can be determined.

Key words: &gamma, -aminobutyric acid； extract； separate； determine；

ZHENG Hong-Fa, HUANG Ya-Hui, HUANG Huai-Sheng, SU Ben-Wen, YUAN Ying-Fang. Study on Extraction and Determination of γ-Aminobutyric Acid in Tea[J]. FOOD SCIENCE, 2008, 29(4): 347-351.

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Study on Extraction and Determination of γ-Aminobutyric Acid in Tea

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