FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (4): 366-369.

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Protective Effect of Pu-erh Tea Extracts on High-glucose-induced Damage in Human Fibroblast Cells

 JIE  Guo-Liang, HE  Pu-Ming, ZHANG  Long-Ze, ZHAO  Bao-Lu, 吕Hai-Peng , LIN  Zhi   

  1. 1.Tea Research Institute, Zhejiang University; 2. State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Acadeny of Sciences; 3.Tea Research Institute, Chinese Academy of Agricultural Sciences
  • Online:2008-04-15 Published:2011-08-24

Abstract: In this study, the Pu-erh tea was successively extracted with acetone, water, chloroform, ethyl acetate, and n-butanol. The extracts were then isolated by column chromatography. Our study demonstrated that both the Fraction 2 (E2 and), Fraction 8 (E8) from ethyl acetate extract, and the Fraction 4 (B4) and, Fraction 5 (B5) from n-butanol extract have strong superoxide anions and hydroxyl radicals scavenging activity. The four fractions showed above have protective effects against high concen- tration glucose-induced loss of HPF-1 cell viability.

Key words: tea, Pu-erh tea, free radicals, natural antioxidants, antioxidant effect, HPF-1 cells