Abstract: Corn flour as mixed carbonic source and bran+peptone+yeast as mixed nitrogenous source,the liquid culture medium of Phellinus linteus mycelia was optimized by orthogonal test in this study.The results showed that the optimal culture medium comprised corn flour 3%,bran 3%,glucose 3%,KH2PO4 0.3%,MgSO4·7H2O 0.15%,VB1 20μg/100 ml,VB2 30μg/100 ml,and on the culture conditions,of culture temperature 32.5 ℃,natural pH,rotation speed 130 r/min and culture cycle 192 h,the average mycelial biomass was 4.4 g/100 ml.

Key words: Phellinus linteus, liquid culture medium, orthogonal test, optimiztion, mycelia, biomass