Abstract: Technologic conditions of whey protein enzymolydrosis for calcium binding peptides production were optimized in this research.Based on the output of calcium binding peptides and content of amino nitrogen,the main factors affecting the enzymolydrosis were experimentally studied in details.Results showed that the content of amino nitrogen could reach 0.272 mg/ml under the optimal conditions of the enzymolydrosis temperature 45 ℃,the time 100 min,the whey protein concentration 7.0 %,substrate to enzyme ratio 70:1,and the solution pH 8.00.On these conditions,the strongest Ca-binding activity was obtained.

Key words: whey protein, hydrolysis condition, calcium binding peptide