Abstract: Objective: To analyze the ingredients in volatile oil extracted from Chrysanthemum moriflium Ramat and to determine the content of β-elemene in the volatile oil. Method: GC- MS analysis was used to analyze the composition of the volatile oil and the ninner standard method was used to determine the content of β-elemene . Results : 59 compounds among 125 peaks separated out by GC-MS were indentified , accounting for 61.74% of total quantity of the volatile oil. The linear range of β- elemene is from 0.006 mg/ml to 0.096 mg/ml. The relative standard derivation( RSD) of repeated experiment is 0.85 % . The average recovery is 95.23 %. And the content of β-elemene in chrysanthemum moriflium Ramat is 12.98 mg/kg. Conclusions: The analysis of main composition of volatile oil from Chrysanthemum moriflium Ramat by GC- MS can enrich the study on constituents of this plant in the world and lay the foundation for further utilization. The internal standard method for determine the content of β-elemene in the volatile oil is simple, accurate, reproducible and practical. This study can promote development and utilization of traditional Chinese herbal medicines.

Key words: Chrysanthemum moritilum Ramat； volatile oil； analysis； &beta, -elemene；