Abstract:

A high performance liquid chromatographic method was developed for the determination of iprodione in fruits. Samples were extracted using acetonitrile, cleaned up by solid phase extraction using PSA and ODS (1:1, m/m) as dispersants. The chromatographic seperation was achieved on an ODS reverse-phase (RP) column. There was a good linear calibration curve in the range from 0.1 to 5.0 μg/ml with the correlation coefficient of 0.9994. The recoveries for iprodione in banana, orange and mango spiked at 0.5, 1.0 and 2.0 mg/kg ranged from 82.70% to 108.62%, with the RSDs of 1.8%－5.3%. The method detection limit was 0.036 mg/kg. This method is simple, sensitive, accurate and reproducible.

Key words: matrix solid phase dispersion extraction, liquid chromatography, iprodione, fruits