Purification and Identification of Peanut Allergen Ara h 6

doi:10.7506/spkx1002-6630-201015017

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (15): 76-80.doi: 10.7506/spkx1002-6630-201015017

• Basic Research • Previous Articles Next Articles

Purification and Identification of Peanut Allergen Ara h 6

LUO Chun-ping1,2,3，GAO Jin-yan4，HU Chun-qiu1,2，CHEN Hong-bing1,2,*，YAN Fei2,3

(1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China ；
2. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China ；
3. School of Environmental and Chemical Engineering, Nanchang University, Nanchang 330047, China；
4. School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330047, China)

Received:2010-04-22 Online:2010-08-15 Published:2010-12-29
Contact: CHEN Hong-bing1 E-mail:chbgjy@hotmail.com

Abstract

Abstract:

In order to effectively isolate and purify peanut allergen Ara h 6, peanut was subjected to a series of sequential treatments, namely de-fatting, protein extraction and anion-exchange chromatographic. Sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE), matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF/MS) and Western blotting were used for the identification of target protein. The results indicated that the purified target protein was peanut allergen Ara h 6 with a molecular weight of 15 kD. The purity of Ara h 6 was more than 95%, and the recovery rate was 22.5%. Based on these investigations, a simple and efficient approach to purifying Ara h 6 is achieved, which will provide experimental materials for further research on peanut allergy.

Key words: peanut allergy, Ara h 6, isolation and purification

CLC Number:

TS201.21

LUO Chun-ping1,2,3，GAO Jin-yan4，HU Chun-qiu1,2，CHEN Hong-bing1,2,*，YAN Fei2,3. Purification and Identification of Peanut Allergen Ara h 6[J]. FOOD SCIENCE, 2010, 31(15): 76-80.

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Purification and Identification of Peanut Allergen Ara h 6

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