Abstract:

The enzymatic hydrolysis of Apostichopus japonicus alimental tract with papain was carried out for polysaccharide extraction, and the obtained polysaccharides were preliminarily characterized. Three hydrolysis parameters including hydrolysis time and temperature as well as enzyme amount were optimized. The relative molecular mass and sulfate group content of the purified product of the crude extract obtained after deproteinization by Sevag method and subsequent Sephacryl S-400 gel filtration chromatography were measured. Enzyme amount of 10400 U/g, hydrolysis temperature of 55 ℃and hydrolysis time of 6 h were found optimal, and the polysaccharide yield was 8.1‰ under these hydrolysis conditions. After Sephacryl S-400 column purification, an acidic polysaccharide with single composition was obtained, and its relative molecular mass might be 25 kD and its sulfate group content was approximately 9.29%.

Key words: Apostichopus japonicus alimental tract polysaccharide, extraction, purification