Abstract:

A pathological mouse model induced by salbutamol was established for evaluating its pathological roles. HE staining of frozen sections showed serious damages in liver tissues, which exhibited scattered distribution rather than radial-arranged central veins in liver leaflets and obvious apoptotic bodies in liver tissues. Simultaneously, coagulation necrosis was also observed in heart tissues due to the coagulation of a large number of myocardial fibers. Moreover, the liver of pathological mice was used as a positive sample to detect its enzymatic hydrolysis using LC-MS/MS. Results indicated that β -D-glucuronic glucosidase or arylsulfatase was the effective enzyme for salbutamol determination and the determination results obtained using enzymatic hydrolysis was three times as high as those obtained without using enzymatic hydrolysis.

Key words: salbutamol, frozen section, HE staining, LC-MS/MS, liver, enzymatic hydrolysis