FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (12): 10-13.doi: 10.7506/spkx1002-6630-201112003

• Processing Technology • Previous Articles     Next Articles

Purification of Proteins from Edible Bird s Nest by Liquid-Phase Isoelectric Focusing

HUANG Xiu-li1,2，LAI Xin-tian1，LIN Lin1，ZHANG Shi-wei1，LIU Xiao-qing1， ZHAN Jia-fen1，LIN Jun-fang2，YANG Guo-wu1,*   

  1. 1. Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518131, China； 2. College of Food Science, South China Agricultural University, Guangzhou 510642, China
  • Online:2011-06-25 Published:2011-06-10

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Abstract: Liquid-phase isoelectric focusing (LIEF) was applied to purify proteins extracted from edible bird s nest (EBN). After LIEF prefractionation, totally 10 fractions were collected and analyzed with SDS-polyacrylamide gel electrophoresis (PAGE). The results showed that gelationus materials mainly concentrated at the first four fractions, and there were also some impurities at the last two fractions. So fractions five through eight were pooled as purified protein sample for following two-dimensional electrophoresis (2-DE) analysis. By using LIEF combined with 2-DE, 4 proteins from EBNs were well separated, and about 30 to 60 2-DE spots were detected on the 2-DE gels. The findings confirmed that LIEF as a prefractionation prior to IPG-IEF can produce the best 2-DE patterns for EBN.

Key words: edible bird s nest (EBN), liquid-phase isoelectric focusing (LIEF), two-dimensional electrophoresis (2-DE)

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