Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption

doi:10.7506/spkx1002-6630-201112030

Abstract

Abstract: The characteristics of adsorption and desorption of onion peel flavonoids on six kinds of macroporous resins were compared. Static adsorption and desorption experiments indicated that X-5 resin was the best resin for the separation and purification of flavonoids from onion peel. The optimal process parameters for purifying onion peel flavonoids with X-5 resin were sample loading flow rate of 0.5 mg/mL, sample pH 5.0 and 50 mL of 80% ethanol of as eluent at a flow rate of 1.0 mL/min. After the purification, the purity of flavonoids from onion peel was increased from 7.95% to 80.78% and could reach up to 94.5% after further triple recrystallization. The developed method is simple and feasible for industrialization.

Key words: macroporous adsorption resin, separation, purification, onion peel, flavonoids

CLC Number:

TS255

XU Huai-de，CHEN Jia，BAO Rong，LIU Kun，LI Jin. Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption[J]. FOOD SCIENCE, 2011, 32(12): 133-138.

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Separation and Purification of Flavonoids from Onion Peel by Macroporous Resin Adsorption

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