Development of A PCR Assay for Detection of Tyramine-producing Enterococcus faecalis and Enterococcus faecium

doi:10.7506/spkx1002-6630-201118037

Abstract

Abstract: Objective: The aim of this study was to develop a PCR assay for the detection of tyramine-producing Enterococcus faecalis and Enterococcus faecium. Methods: The tyrosine decarboxylase genes (tdc gene) of Enterococcus faecalis and Enterococcus faecium were compared with those published in GenBank database, and specific primers were designed based on their nonconservative sequences to develop a PCR assay. Results: Each primer pair could only amplified their target gene from the associated ones of 27 bacterial strains tested. The detection limit of this assay was 1.0×102 CFU/mL. Conclusion: The developed PCR assay has good specificity, stability and sensitivity.

Key words: Enterococcus faecalis, Enterococcus faecium, tyrosine decarboxylase gene, polymerase chain reaction (PCR)

CLC Number:

TS207.4

SHU Rui-hua，LU Shi-ling，XU Xing-lian. Development of A PCR Assay for Detection of Tyramine-producing Enterococcus faecalis and Enterococcus faecium[J]. FOOD SCIENCE, 2011, 32(18): 176-179.

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Development of A PCR Assay for Detection of Tyramine-producing Enterococcus faecalis and Enterococcus faecium

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