Composition Analysis of Flavonoids from Semen Cuscutae and Antioxidant Activity in Edible Oil and Fat

Abstract

Abstract: Flavonoids from Semen Cuscutae including rutin, quercetin and kaemperferol were determined qualitatively and quantitatively by ultraviolet spectroscopy and high performance liquid chromatography (HPLC). Using rutin as standard substance, flavonoid extract of Semen Cuscutae was determined and found to show two absorption peaks in the range of 300—380 nm and 240—280 nm. The contents of three flavonoid components were determined accurately by HPLC with repeatable results. Flavonoids from Semen Cuscutae displayed strong antioxidant activity in lard and peanut oil in a dose-dependent manner as evaluated based on peroxide value. In both matrices, the antioxidant effect at doses of 0.08% and 0.06% was superior to that of 0.02% BHT and 0.01% PG.

Key words: Semen Cuscutae, flavonoids, rutin, antioxidant activity

CLC Number:

TS209

CHEN Lin-lin. Composition Analysis of Flavonoids from Semen Cuscutae and Antioxidant Activity in Edible Oil and Fat[J]. FOOD SCIENCE, 2012, 33(15): 103-106.

References

[1] 郭洪祝, 李家实. 南方菟丝子黄酮类成分的研究[J]. 中国中药杂志, 1997, 22(1): 38-39.
[2] 张崇高. 我国菟丝子研究概况[J]. 杂草科学, 2003, 1: 1-4.
[3] 张晓鹤. 菟丝子的研究进展[J]. 天津药学, 2004, 4: 43-45.
[4] 叶敏. 菟丝子药理研究进展[J]. 北京中医药大学学报, 2000, 23(9): 52-53.
[5] 钟秋平, 林美芳. 黄皮果中总黄酮含量的测定及其黄酮种类的鉴别[J]. 食品科学, 2007, 8: 411-413.
[6] 张鞍灵, 高锦明. 黄酮类化合物的分布及开发利用[J]. 西北林学院学报, 2000, 15(1): 69-74.
[7] Hyo-Joung Seo, Young-Joon Surh.Eupatilin. A pharmacologically active flavone derived from Artemisia plants, induces apoptosis in human promyelocytic leukemia cells[J].J Mutation Research, 2001, 496: 191- 198.
[8] 叶敏，阎玉凝，乔梁等. 中药菟丝子化学成分研究[J]. 中国中药杂志, 2002,, 27(2): 115-117.
[9] 徐清. 中药菟丝子的研究进展[J]. 河南中医药学刊, 2001, 9: 79-80.
[10] 宋小妹, 张培芳. 银杏叶总黄酮提取分离工艺研究[J]. 山西中医药学院学报, 1998, 21(2): 34-36.
[11] 赵翾, 李红良, 张惠妹. 响应面法优化火麻仁黄酮提取工艺[J]. 食品科学, 2011, 1: 127-131.
[12] 彭游, 叶志刚, 邓泽元. 沙棘黄酮光波干法辅助提取及其机理研究[J]. 2011, 4: 197-200.
[13] 李维莉, 马银海. 蜂胶黄酮提取方法研究[J]. 食品科学, 2003, 24(5): 100-101
[14] 潘见, 骆祥峰. 紫外分光光度法测银杏黄酮干含量误差分析[J]. 合肥工业大学学报, 2000, 23(4): 464-467.
[15] Min Ye, Yan Li. Determination of Flavonoids in Semen Cuscutae by RP-HPLC[J]. J Pharm and Bio Analysis, 2002, 28: 621-628.
[16] 刘书成, 李元瑞. 大蒜精油对食用油脂的抗氧化特性[J]. 食品科学, 2002, 23(1): 128-131.
[17] 张德海, 杨晓泉. 柚皮提取物的抗氧化作用研究[J]. 中国油脂, 2001, 26(4): 54-57.