Extraction and Bioactivity of Triterpenoids from Fruit Bodies and Mycelia of Inonotus obliquus

Abstract

Abstract: In order to explore the difference in medicinal value of cultured mycelia and fruit bodies of Inonotus obliquus, organic solvent cold soaking and ultrasonic reflux were adopted to extract triterpenoids from fruit bodies and mycelia of Inonotus obliquus. The triterpenoids extracted from both sources were determined to be the same or similar homologues by thin layer chromatography (TLC) and Fourier transform infrared (FT-IR) spectral analysis. Both triterpenoid extracts could inhibit the growth of cancer cells and bacteria as determined by tetrazolium salt assay (MTT) and cylinder plate method. The highest inhibitory rate of triterpenoids to human gastric cancer cell line MGC-803 reached up to 38.27%. Therefore cultured mycelia of Inonotus obliquus have the same medicinal value as fruit bodies.

Key words: Inonotus obliquus, triterpenes, anti-tumor activity, anti-bacterial activity

CLC Number:

Q541

References

[1] 李建光, 司俊娜, 高愿军. 桦褐孔菌研究综述[J]. 安徽农业科学, 2008, 36(2): 571-572, 582.
[2] Hyun K.W., Jeong S.C., Lee D.H., et al. Isolation and characterization of a novel plateletag gregation inhibitory peptide from the medicinal mushroom, Inonotus obliquus[J]. Peptides, 2006, Vol. 27: 1173-1178.
[3] Kim Y.O., Han S.B., Lee L.W., et al. Immuno-stimulating effect of the endopolysaccharide produced by submerged culture of Inonotus obliquus[J]. Life Sciences., 2005, Vol. 77: 2438-2456.
[4] 陈艳秋，李玉．桦褐孔茵的研究进展[J]．微生物学通报， 2005， 32(2)： 124-127.
[5] 潘春丽, 查勇, 张鑫, 等. 桦褐孔菌三萜化合物提取工艺的优选[J]. 生物加工过程, 2008, 6(4): 36-39.
[6] 周俐斐, 芦柏震, 侯桂兰. 中药三萜类化合物的提取分离及测定方法研究进展[J]. 中药与天然药物, 2007, 19(3): 62-65.
[7] 沈思, 李孚杰, 梅光明, 等. 茯苓皮三萜类物质含量的测定及其抑菌活性的研究[J]. 食品科学, 2009, 30(1): 95-98.
[8] 李淼, 鲁战会，孔晓玲，等. 野生与人工栽培桦褐孔菌提取物抗糖基化终产物及降血压活性比较[J]. 食品科技， 2010, 35（11）: 198-202.
[9] 田明月, 俞力,徐秀泉. 正交设计法优化桦褐孔菌总三萜超声提取工艺[J]. 中国实验方剂学杂志, 2011, 17(12): 31-33.
[10] 沈蕾. 细胞凋亡与肿瘤的关系[J]. 临床儿科杂志, 2002, 20(l0): 638-640.
[11] 刘高强, 章春莲, 彭广生. 赤芝菌体中三萜抑菌作用研究[J]. 时珍国医国药, 2008b ,19( 11): 2578-2579.
[12] 李英秀, 崔基成, 孔东植. 桦褐孔菌提取物对胃癌MGC-803细胞株的抗增殖与诱导作用[J]. 菌物研究, 2003, 1(1): 17-23.
[13] 孙常松, 李玛琳. 五环三萜类化合物抗肿瘤活性及其机制研究进展[J]. 中国民族民间医药, 2009,18(12):14-15.
[14] Weifa Zheng, Meimei Zhang, Yanxia Zhao,et al. Accumulation of antioxidant phenolic constituents in submerged cultures of Inonotus obliquus[J]. Bioresource Technology 100 (2009) 1327–1335.
[15] Wasser S P. Medicinal mushrooms as a source of antitumor and immunmodulating poly saccharrides [J]. Apple Microbool Biotechnol, 2002, 60: 258.
[16] 卢丹, 刘金平, 李平亚. 三萜类化合物抗癌活性研究进展[J]. 特产研究, 2010,1(1): 65-68.
[17] Yana Song. Jing Hui,Wei Kou, et al. Identification of Inonotus obliquus and Analysisof Antioxidation and Antitumor Activities of Polysaccharides[J].Current Microbiology, 2008, 57(5): 454-462.