Digestion Characteristics in vitro of Phycobiliprotein from Nostoc sphaeroides Ku..ting

Abstract

Abstract: In this study, some properties of products produced during digestion of Nostoc sphaeroides ku..ting phycobiliprotein in simulated gastric and intestinal fluids were investigated especially such as soluble protein and free amino acid contents and antioxidant properties. Meanwhile, the change in molecular weight during digestion of Nostoc sphaeroides ku..ting phycobiliprotein was determined by HPLC. The results showed that protein content revealed a downward trend during digestion in each stimulated fluids, and consequently free amino acid content exhibited an upward trend. Digestion products had stronger ABTS radical scavenging and anti-lipid peroxidation activities but weaker hydroxyl radical scavenging activity in D-deoxyribose system than Nostoc sphaeroides ku..ting phycobiliprotein. The results of partial correlation analysis demonstrated that 6 ku and 0.2 ku peptides were closely related to the antioxidant effect of digestion products of Nostoc sphaeroides ku..ting phycobiliprotein.

Key words: Nostoc sphaeroides Ku..ting, phycobiliprotein, simulated gastric and intestinal fluids, antioxidant activity, HPLC

CLC Number:

TS201.1

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