FOOD SCIENCE ›› 2012, Vol. 33 ›› Issue (15): 99-102.

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Microstructural Observation of Leucaena glaucal Seed Polysaccharides under Atomic Force Microscope

  

  • Received:2011-06-25 Revised:2012-06-24 Online:2012-08-15 Published:2012-09-07
  • Contact: Yong-Li YANG E-mail:rose_kally@yahoo.com.cn

Abstract: Objective: The microstructure of Leucaena glaucal seed polysaccharides was studied based on morphological observation under atomic force microscope (AFM). Methods: Polysaccharides from were extracted by hot water extraction. The structure and morphology of aggregates and non-aggregates from Leucaena glaucal seed polysaccharides formed through varying polysaccharide concentration or chemically modifying the surface of mica substrates were visualized under AFM. Results: Membranous, granular aggregates structure and non-aggregate structure of a single polysaccharide molecule could be observed in the samples prepared at various conditions. Conclusion: A single Leucaena glaucal seeds polysaccharide molecule was linear spiral, and had short branch structure and entangled molecular chains to form a reticulation structure.

Key words: atomic force microscope, Leucaena glaucal seed polysaccharide, microstructure

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