Abstract: This work reports the application of phase separation for the separation of alanine produced with halogenated carboxylic acid. The purification of crude alanine with washing solution at a solid-to-solvent ratio of 1:16 was carried out under the condition of 20 ℃ to investigate the effect of number of washing cycles on the removal rate of ammonium chloride and the recovery and purity of alanine. Over 88.0% of the impurity ammonium chloride was removed after the tenth washing cycle and the purity and average recovery of alanine were larger than 98.0% and equal to 93.6%, respectively. The process of phase separation was easy, effective and suitable for the separation and purification of alanine produced by halogenated carboxylic acid method.

Key words: alanine, phrase separation, ammonium chloride, purification