Optimization of Reflux Extraction Process for Total Flavonoids from Cupressus chengjana S. Y. Hu by Response Surface Methodology

Abstract

Abstract: Objective: To optimize the reflux extraction of total flavonoids from leaves and stems of Cupressus chengjana S. Y. Hu. Methods: One-factor-at-a-time design and response surface methodology (RSM) were used to determine optimal conditions for the extraction of total flavonoids through regression analysis of the relationship between the extraction efficiency of total flavonoids and various process parameters. Results: The optimal extraction process was soaking for 37 min in 70% ethanol solution at a solid-to-solvent ration of 1:40 (g/mL) followed by reflux extraction repeated 3 times for 70 min each time, resulting in an extraction efficiency of 7.211%. Conclusion: Response surface methodology can provide an accurate and convenient way to predict the reflux extraction of total flavonoids to obtain optimal process with high effectiveness and applicability.

Key words: response surface methodology (RSM), Cupressus chengjana S. Y. Hu, total flavonoids, reflux extraction

CLC Number:

TQ461

Mei YANG. Optimization of Reflux Extraction Process for Total Flavonoids from Cupressus chengjana S. Y. Hu by Response Surface Methodology[J]. FOOD SCIENCE, 2012, 33(18): 57-62.

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