The Effects of Bioactive Plant Compounds on Cell viability and Comparison of Different Measuring Methods

Abstract

Abstract: Resveratrol and chlorogenic acid, as phytochemicals were used to treat CHO cells. Cell viability was measured by using MTS, WST-1, CCK-8, XTT and MTT methods and the optimal method from MTS, WST-1, CCK-8 and XTT was screened by comparing with the conventional MTT methods. The cytotoxicity of resveratrol and cholorogenic was compared by IC50 value. Results: Compared with MTT assay, CCK-8 assay was timesaving, repeatable and easily operated. IC50 of resveratrol and chlorogenic acid detected by CCK-8 were closed to one by MTT assay. Thus, CCK-8 assay was optimal. On the contrary, WST-1 assay was less repeatable and cell viability rate tended to smaller. XTT assay was the worst because of its instability in wavelength, experimental time and repetition. IC50 of resveratrol on CHO cells were 116.86、124.68、64.90、329.83、86.30 μmol/L in CCK-8、MTS、WST-1、XTT and MTT assay. While IC50 of chlorogenic acid on cells were 2808.60、1119.96、1029.72、1325.43、2852.63 μmol/L respectively. The results showed higher IC50 value for chlorogenic acid. Conclusions: CCK-8 assay was a optimal method for cell viability ; Resveratrol showed higher cytotoxicity to CHO cells compared with chlorogenic acid.

CLC Number:

Q946
R994.4

References

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