Screening of Antioxidant Components from Ledum palustre L. Leaves by Online HPLC-ABTS+ Method

Abstract

Abstract: In this study, antioxidant compounds from Ledum palustre L. leaves were screened and identified by using an online high-performance liquid chromatography (HPLC)-ABTS+· screening system. Two antioxidant compounds from the ethyl acetate fraction in methanol extract were obtained. By mass spectroscopy (MS) and nuclear magnetic resonance (NMR) spectroscopy, both compounds were identified as genistin and quercetin, respectively.

Key words: Ledum palustre L., HPLC-ABTS+·, screening system, online detection, antioxidant activity, NMR, MS

CLC Number:

R446.61

References

[1]He Wenhao, Liu Xuan, Xu Honggao, et al.On-line HPLC-ABTS screening and HPLC-DAD-MS/MS identification of free radical scavengers in Gardenia (Gardenia jasminoides Ellis) fruit extracts[J].Food Chemistry,2010,123(2):521-528
[2]Li Hea, XUA Honggao, LIU Xuan.Identification of phenolic compounds from pomegranate (Punica granatum L) seed residues and investigation into their antioxidant capacities by HPLC–ABTS+ assay[J].. Food Research International,2011,44(5):1161-1167
[3]Osman A.M.，Wong, KK.Y.，Fernyhough A. ABTS radical-driven oxidation of polyphenols isolation and structural elucidation of covalent adducts[J].. Biochemical and Biophysical Research Communications,2006,346:321-329
[4]Shang Yafang, Sangmin Kim and ByunghHun Um et al.Pressurized liquid method for fucoxanthin extraction from Eisenia bicyclis (Kjellman) Setchell[J].Journal of Bioscience and Bioengineering,2011,111(2):237-241
[5]Dutta N K，Mazumdar K, et al.Isolation and identification of a flavones(Quercetin) from Butea frondosa bark[J].Phamaceutical Chemistry Journalm,2007,41(5):37-39
[6]Lin Yuying, Kong Lingyi.Studies on the Chemical Constituents of Desmodium styracifolium (Osbeck) Merr[J].Asian Journal of Traditional Medicines,2006,1(1):34-37
[7]关爽. 杜香提取物的制备及部分毒理药理作用[J].吉林大学,2009,:-
[8]韩光亮，李翠梅，Eduardo Cacace 等.改良的ABTS+法及其在优化抗氧化活性物质提取中的应用[J].卫生研究,2004,33(5):620-623
[9]黄莹. 细叶杜香的化学成分研究[J].广东药学院,2008,:-
[10]林恋竹，赵谋明.反应时间对DPPH法、ABTS法评价抗氧化性结果的影响[J].食品科学,2010,31(5):63-67
[11]耿雪飞，郑永杰，赵明，UM Byunghun.基于HPLC-ABTS体系筛选细叶杜香抗氧化活性成分[J].化学工程师,2011,10:70-73
[12]马雪梅.资源植物杜香的研究进展[J].安徽农业科学,2010,6:2949-2950
[13]马雪梅. 大兴安岭地区杜香有效成分的研究[J].东北林业大学,2004,:-
[14]张敏. 杜香化学成分及抗炎止痛作用研究[J].华东师范大学,2009,:-
[15]王炎，赵敏.大兴安岭地区细叶杜香挥发性成分的研究[J].色谱,2003,2(6):631-631
[16]杨华，韩坤，宛晓春，方从兵.野葛异黄酮糖苷的分离纯化及体外清除自由基活性的研究[J].安徽农业大学学报,2011,38(2):146-150