Hydrolysis Kinetics of Bovine Bone Collagen by Bacillus cereus Extracellular Collagenase

Abstract

Abstract: In order to explore the hydrolysis kinetics of bovine bone collagen by collagenase isolated from Bacillus cereus MBL13 and the optimal hydrolysis conditions, the effects of substrate concentration, hydrolysis temperature, enzyme concentration, hydrolysis pH and hydrolysis time on the degree of hydrolysis (DH) were analyzed. The optimal conditions for the hydrolysis of bovine bone collagen were bone collagen concentration of 30 g/L, hydrolysis time of 6 h, hydrolysis temperature of 45 ℃, initial enzyme concentration of 0.35 g/100 mL and hydrolysis pH of 8.0. Based on the experimental data, a kinetic model equation was obtained as V = 9.3633·[S]/(114.785+[S]). Enzymatic hydrolysis process was analyzed at the wavelength of 230 nm and 570 nm.

Key words: collagenase, bovine bone collagen, collagen polypeptide, enzymatic hydrolysis, kinetics

CLC Number:

TS251

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