Construction of a Strain of Recombinant Lactococcus lactis Expressing Food-Grade Nattokinase

Abstract

Abstract: An integrative expression vector designated as pFY008 was constructed with HisH gene as a homologous recombination target site. For food-grade expression of nattokinase gene (aprN) in Lactococcus lactis, the expression cassette PnisA-aprN gene was integrated into the genome of Lactococcus lactis by homologous recombination. A food-grade recombinant Lactococcus lactis was constructed to produce a thrombolytics protein. The recombinant Lactococcus lactis was found to have fibrinolytic activity. Meanwhile, it had fine stability and high safety.

Key words: nattokinase, homologous recombination, Lactococcus lactis

CLC Number:

Q786

References

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