Effect of Dihydromyricetin on Exercise-induced Fatigue in Mice

Abstract

Abstract: Objective: To explore the anti-fatigue effect of dihydromyricetin (DMY) in mice. Methods: A total of 40 male mice were randomly divided equally into high-dose DMY group (300 mg/kg), middle-dose DMY group (200 mg/kg), low-dose DMY group (100 mg/kg) and control group (distilled water). The mice were intragastrically administered the same volume of DMY solution or distilled water once daily for one successive week. Anti-hypoxia and weight-bearing swimming tests were carried out to analyze changes in serum lactic acid and urea nitrogen contents and liver glycogen content, MDA content and SOD activity before and after exercise. Results: Compared with the control group, weight-bearing swimming time was significantly prolonged by DMY administration at three doses, while a significant prolongation in anoxia survival time was observed for only high-dose and middle-dose groups (P＜0.05); in addition, both groups revealed a significantly higher level of liver glycogen (P＜0.01) but significant lower levels of serum lactic acid and urea nitrogen (P＜0.05 or P＜0.01) and liver MDA (P＜0.01), whereas there was no significant difference in SOD activity between the two DMY groups. Conclusion: DMY has an obvious anti-fatigue effect, which may be related to the inhibition of lipid peroxidation in liver tissue.

Key words: dihydromyricetin, exercise-induced fatigue, weight-bearing swimming, anti-anoxia, antioxidation

CLC Number:

TS275.4

References

[1] 国家中医药管理局《中华本草》编委会. 中华本草(第5册)[M]. 上海:上海科学技术出版社, 1999: 279.
[2] Qi Shimei, Xin Yinqiang, Guo Yingtao, et al. Ampelopsin reduces endotoxic inflammation via repressing ROS-mediated activation of PI3K/Akt/NF-κB signaling pathways[J]. Int Immunopharmacol. 2012, 12(1): 278-287.
[3] 朱哲, 杨悟新, 强烈应, 等. 二氢杨梅素研究进展[J]. 武警医学院学报, 2011, 20(7): 600-604.
[4] 何来英, 严卫星, 楼密密, 等. 保健食品抗疲劳作用试验方法研究[J]. 中国食品卫生杂志, 1997, 9(4): 1-6.
[5] 祖元刚, 杨慧洁, 张琳, 等. 红甜菜鲜汁抗疲劳作用的实验研究[J]. 食品科学, 2011, 32(1): 215-217.
[6] 孙睿, 李秀霞, 罗志文, 等. 榛仁多糖对小鼠抗疲劳及耐缺氧能力的影响[J]. 食品科技, 2010, 35(7): 59-61.
[7] 张海信. 运动性疲劳之代谢产物堆积学说研究进展[J]. 内蒙古体育科技, 2005, 18(1): 22-24.
[8] Stackhouse SK, Reisman DS, Binder-Macleod SA. Challenging the role of pH in skeletal muscle fatigue[J]. Phys Ther. 2001, 81(12):1897-1903.
[9] DE PAULA BROTTO M, VAN LEYEN SA, BROTTO LS, et al. Hypoxia/fatigue-induced degradation of troponin I and troponin C: new insights into physiologic muscle fatigue[J]. Pflugers Arch. 2001, 442(5):738-744.
[10] GERMINARIO E, ESPOSITO A, MEGIGHIAN A, et al. Effects of modulators of sarcoplasmic Ca2+ release on the development of skeletal muscle fatigue[J]. J Appl Physiol. 2004, 96(2):645-649.
[11] WALSH ML. Whole Body Fatigue and Critical Power: A Physiological Interpretation[J]. Sports Medicine, 2000, 29(3):153-166.
[12] 覃潇, 韦玉娜. 二氢杨梅素活血化瘀作用的实验研究[J]. 中国中医药科技, 2011, 18(1): 36-38.
[13] TAKEDA K, MACHIDA M, KOHARA A, et al. Effects of citrulline supplementation on fatigue and exercise performance in mice[J]. J Nutr Sci Vitaminol (Tokyo). 2011, 57(3):246-250.
[14] 潘照斌, 李棐朝, 廖月娥, 等. 二氢杨梅素抗血栓形成的作用研究[J]. 中医药导报, 2010, 16(11): 92-94.
[15] 张秀桥, 沈伟, 陈树和, 等. 大叶蛇葡萄提取物对肾性高血压大鼠降压作用的实验研究[J]. 中国医院药学杂志, 2008, 28(24): 2095-2096.
[16] WESTERBLAD H, ALLEN DG. Emerging roles of ROS/RNS in muscle function and fatigue[J]. Antioxid Redox Signal. 2011, 15(9): 2487-2499.
[17] IBRAHIM MY, ASHOUR OM. Changes in nitric oxide and free radical levels in rat gastrocnemius muscle during contraction and fatigue[J]. Clin Exp Pharmacol Physiol. 2011, 38(12): 791-795.
[18] Dutta A, Ray K, Singh VK, et al. L-carnitine supplementation attenuates intermittent hypoxia-induced oxidative stress and delays muscle fatigue in rats[J]. Exp Physiol. 2008, 93(10):1139-1146.