Separation, Purification and Stability of Theaflavins

Abstract

Abstract: Theaflavins have attracted extensive attention due to their strong bioactivity. The instability and easy degradation in aqueous solution of theaflavins limit their applications. Theaflavin monomers with high purity (＞95%) including theaflavin (95.6%), theaflavin-3-O-gallate (95.2%), theaflavin-3’-O-gallate (99.8%) and theaflavin-3,3’-O-gallate (96.3%) were separated from a sample of commercial theaflavin mixture. Their stability under various environmental conditions (pH, temperature and medium composition) was investigated. It was found that higher concentrations of theaflavins were more stable during storage at lower temperatures and pH levels, thereby being beneficial to the preservation of quality and the extension of shelf life.

Key words: theaflavins, diol-silicon gel, preparative chromatography, stability

CLC Number:

Q914

References

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