Effect of Steam Explosion Intensity on Xylo-oligosaccharide Production by Enzymatic Hydrolysis of Corncobs

Abstract

Abstract: The production of xylo-oligosaccharides (XOS) from corncobs was performed by steam explosion pre-treatment combined with selectively enzymatic hydrolysis with endo-xylanase. A new precise quantification method for XOS with a degree of polymerization (DP) of 2–6 was developed using high performance anion-exchange chromatography coupled with pulsed amperometric detector (HPAEC-PAD). The effects of pretreatment intensity and major parameters (reaction temperature and reaction time) on the composition distribution and yield of XOS were investigated in order to find optimal steam explosion pretreatment conditions. The results indicated that the optimal explosion intensity factor, temperature and holding time for steam explosion pretreatment of corncobs were 3.76, 200 ℃ and 390 s, respectively. Under these conditions, the highest yield of XOS was 20.8%. The resulting product was mainly composed of xylotriose, xylotetraose and xylobiose with a relative content of 43.5%, 21.3% and 18.6%, respectively.

Key words: xylo-oligosaccharides (XOS), steam explosion pretreatment, selectively enzymatic hydrolysis, corncobs, high performance anion-exchange chromatography coupled with pulsed amperometric detector (HPAEC-PAD)

CLC Number:

TQ353.6

References

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