Extraction and Antioxidant Activity of Flavonoids from Gynura crepidioides

Abstract

Abstract: The extraction of flavonoids from Gynura crepidioides stems and leaves was optimized using an L9(34) orthogonal array design. The effects of ethanol concentration, temperature, extraction time and solid/solvent ratio on extraction efficiency were examined. The optimal extraction conditions were found to be extraction at 65 ℃ for 3.5 h with 70% ethanol with a solid/solvent ratio of 1:30 (g/mL). Under these conditions the measured extraction yield of flavonoids from Gynura crepidioides stems and leaves was 31.88 mg/g. The obtained extract had a considerable scavenging effect against hydroxyl free radicals in a dose-dependent fashion. Besides, it also could scavenge superoxide anion free radicals. Based on these results, it can be concluded that flavonoids from Gynura crepidioides stems and leaves have the potential to be developed as promising free radical scavengers.

Key words: Gynura crepidioides, flavonoids, extraction, antioxidant activity

CLC Number:

R931.6

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