Abstract: Obiective: To separate and identify chemical constituents in the stems and leaves of Fallopia aubertii (L. Henry) Holub. Methods: The dried stems and leaves of the plant were extracted repeatedly with 95% methanol for four times, and then the pooled extract was re-extracted sequentially with ethyl acetate and n-butanol. At last, the two fractions obtained were separated using various column chromatographic techniques. In addition, the structure of the separated fractions was identified and elucidated according to their physicochemical properties and spectral data as well as relevant literature data. Results: Eleven compounds were isolated and identified as 5,7-dihydroxy-2-(n-heneicosyl) chromone (1), quercetin (2), kaempferol (3), luteolin (4), myricetin (5), myricetin-3-O-β-D-glucopyranoside (6), quercetin-3-O-α-L-rhamnoside (7), N-feruloyltyramine (8), p-coumaroyldopamine (9), β-sitosterol (10) and daucosterol (11), respectively. Conclusions: It is the first time that compounds 1, 5, 6 and 9 are isolated from the genus Fallopia and all identified compounds except compound 10 from Fallopia aubertii (L. Henry) Holub.

Key words: Fallopia aubertii (L. Henry) Holub, chemical constituents, isolation and identification