Optimization of Cryoprotectant Formulation for Candida tropical by Central Composite Design

Abstract

Abstract: A cryoprotectant formulation consisting of sucrose, L-sodium glutamate and polyethylene glycol for Candida tropical was optimized using a response surface central composite design. By regression analysis of the experimental data obtained, the optimum cryoprotectant formulation was found to consist of 14.15 g/100 mL sucrose, 7.07 g/100 mL L-sodium glutamate and 1.01 g/100 mL polyethylene glycol. The predicted survival rate of Candida tropical with the addition of the optimized cryoprotectant formulation after freeze-drying was 81.46%, which was close to the actual value of 82.73%.

Key words: Candida tropical, cryoprotectant, central composite design (CCD), response surface methodology (RSM)

CLC Number:

TS201.3

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