Abstract:

Objective: To explore the in vitro antioxidant activity of functional oligosaccharide from Stachys sieboldii
Miq. and its effect on the in vitro proliferation of mouse lymphocytes. Methods: The reducing power, scavenging
capacity against hydroxyl, superoxide anion and DPPH radicals of the functional oligosaccharide and its effect to
promote the proliferation of peripheral blood lymphocytes were determined by prussian blue, salicylic acid, pyrogallol
autoxidation, DPPH, and methyl thiazolyl tetrazolium colorimetric methods, respectively. Results: The functional
oligosaccharide had little reducing power and different scavenging effects on these radical systems. Its scavenging
activity against •OH and DPPH free radical was equal to VC at 2.4 mg/mL and the highest levels were 82.0% and
89.3%, respectively. Its scavenging activity against O2
－· was weaker than that of VC. It could significantly stimulate
the proliferation of peripheral blood lymphocytes and showed a synergistic effect with ConA in a dose-dependent
manner. Conclusion: The functional oligosaccharide from Stachys seiboibi has antioxidant activity and can promote the
proliferation of peripheral blood T lymphocytes.

Key words: Stachys seiboibi, functional oligosaccharide, antioxidant activity, peripheral blood T lymphocyte