RSM of Optimized Cellulase Extraction of polyphenol from Carga cathayensis Sarg peels

Abstract

Abstract:

Response surface methodology was employed to optimize the extraction condition of polyphenols from carga cathayensis sarg peels treated with cellulase. On the basis of one-factor-at-a-time experiments, a four-factor, three-level quadratic general rotary unitization design was employed to establish a quadratic polynomial mathematical model that describes extracting rate as a function of four process conditions, and the established model was analyzed by drawing response surface and contour plots. The optimal extraction conditions were determined as follows: 4U/g substrate at pH 6 and 70℃ for 70min. Under these conditions, the extracting rate of carga cathayensis sarg peels was predicted to 63.43% and observed to 61.72% with a relative error of 2.77%.

CLC Number:

R151.3

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RSM of Optimized Cellulase Extraction of polyphenol from Carga cathayensis Sarg peels

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